ALPHA 1 PERFORMANCE LAB · SCIENCE & MOVEMENT DIAGNOSTICS
TECHNOLOGY PARTNER: PNOĒ
GOLD STANDARD CLINICAL CARDIOPULMONARY ANALYSIS

METABOLIC ASSESSMENT

Stop guessing your heart rate zones and caloric burn. Powered by the medical-grade PNOĒ metabolic analyzer, Alpha 1 Performance Lab provides the same clinical breath-by-breath gas exchange testing utilized by Olympic training centers, elite cardiologists, and human longevity institutes.

PNOĒ

Official technology partner for clinical breath-by-breath cardiopulmonary exercise testing (CPET), resting metabolic rate (RMR), and cellular longevity analytics.

PNOE Metabolic Breath Analysis and VO2 Max testing with coach and athlete in real-time tracking
Real-Time Spirometry & Biomarker App Feedback
Gold Standard CPET
Why Breath Analysis Matters

Your Breath Is The Ultimate Biomarker

Every calorie you burn requires oxygen and yields carbon dioxide. By analyzing your precise O2 consumption and CO2 production second by second during rest and ramped exercise, PNOĒ exposes mitochondrial fitness, biological age, metabolic flexibility, and cellular energy efficiency.

23+
Biomarkers Measured In Every Single Breath
Zone 1-5
Individualized Heart Rate Training Zones
Cyclist Wattbike PNOE VO2 Max assessment
Multi-Modal Testing
Treadmill, Wattbike Ergometer & RMR

Tailored to your sport—run, cycle, row, or measure resting metabolism in reclined comfort.

WHOLE-BODY PHYSIOLOGICAL MAPPING

What PNOĒ Assesses Inside Your Body

Breath-by-breath spirometry provides direct diagnostic windows into your vital organ systems, cellular mitochondria, posture mechanics, and central nervous system.

PNOE Brain Function & Posture Diagnostics
Neurological & Biomechanical

Brain Function & Posture

Evaluates autonomic nervous system balance, respiratory sinus arrhythmia, and how spinal posture impacts breathing volume and thoracic ribcage expansion. Identifies hyperventilation tendencies and fight-or-flight sympathetic overdrive.

Autonomic stress & spinal thoracic mechanics
PNOE Lungs & Heart Cardiopulmonary Diagnostics
Cardiopulmonary Engine

Lungs & Heart Capacity

Measures peak cardiac stroke volume, arterial oxygen extraction, and pulmonary gas diffusion. Reveals whether your heart or your lungs are the limiting factor during high-intensity training or endurance efforts.

Cardiac output & pulmonary gas exchange
PNOE Metabolism & Cellular Health Diagnostics
Mitochondrial Bioenergetics

Metabolism & Cellular Health

Maps mitochondrial density, respiratory quotient (RQ), and substrate oxidation ratios. Pinpoints the exact gram-per-hour rate of fatty acid vs. glycogen burning across every heart rate bracket.

Fat oxidation rate & mitochondrial efficiency
FROM METABOLIC DATA TO ACTIONABLE RESULTS

The PNOĒ Prescription Engine

How clinical breath data converts into personalized training zones, macronutrient targets, and breathwork protocols to reverse metabolic slowdown and optimize longevity.

PNOE Analyzes with Lab Precision -> Determines -> In Order To Framework
End-To-End Analysis

Lab Precision to Target Outcomes

Pinpoint VO2 max for Zone 4/5 longevity training, metabolic rate for exact calorie needs, and resting/exercise substrate burn for customized macro and Zone 2 programs.

Reduces biological age & enhances cellular fitness
PNOE Personalized Zone 2 and Zone 4/5 Training Benefits
Cardio & Resistance Matrix

Personalized Zone 2, 4 & 5 Training

Zone 2 training improves fat-burn efficiency, enhances cellular mitochondria, and lowers obesity risk. Zone 4 & 5 training boosts peak VO2 max, enhances lung function, and balances resistance training with cardio.

Reverses metabolic slowdown & maximizes muscle development
PNOE Personalized Nutrition Macros, Calories & Breathwork
Dietary & Neurological Fuel

Macro Intake & Breathwork Protocols

Personalized fat, carb, and protein intake lowers hunger, elevates energy levels, and accelerates fat loss. Clinical breathwork prescriptions target autonomic balance and elevate mood and cognitive focus.

Balances hormones, elevates mood & increases lean muscle
CLINICAL PROTOCOL RECOMMENDATION ENGINE

Personalized Protocol Recommendation Calculator

Select up to 4 areas you’d most like to improve. You don’t need to know exactly what you need — just tell us what you’re experiencing or what you’d like to feel, change, or accomplish. We’ll use your selections to help guide you toward the training, testing, recovery, nutrition, or wellness options that may best fit your goals.

Selected:0/ 4 max
400 areas to improve
2. Tailored Protocol Prescription

Multi-disciplinary recommendations across Lab Diagnostics, Medical Therapies, and Personal Coaching.

Select 1 to 4 Areas to Generate Your Protocol

Choose any combination of areas above (weight loss, recovery, joint stiffness, low energy, athletic performance, etc.) to see how Alpha 1 Performance Lab, Alpha 1 Labs, and Alpha 1 Personal Training coordinate your options.

CLINICAL REPORT SAMPLE

What Does a PNOĒ Metabolic Report Look Like?

Preview the comprehensive 24+ page cardiopulmonary diagnostic packet delivered after your testing session. Includes cellular lung diagnostics, precise fat-burning heart rate zones, and customized nutrition macros.

PNOĒ Official Partner
PHYSIOLOGICAL INSIGHTS

What PNOĒ Reveals About Your Body

Comprehensive data points that transform cardiovascular training from random cardio into scientific protocol.

VO2 Max & Aerobic Capacity

The single strongest clinical predictor of cardiovascular longevity and all-cause mortality, quantified to medical precision via breath-by-breath gas exchange.

Metabolic Crossover & Fat Burn

Maps the precise heart rate thresholds where your mitochondria shift from burning stored fatty acids to glucose/glycogen, optimizing fat loss without muscle wasting.

Resting Metabolic Rate (RMR)

Uncovers your true basal caloric burn at rest—taking the guesswork out of dietary deficits, surplus macros, and plateau breakthroughs.

Respiratory & Lung Mechanics

Measures tidal volume, breathing frequency, and carbon dioxide exhalation efficiency to uncover respiratory limitations restricting athletic output.

TWO TESTING MODALITIES

RMR vs. Active VO2 Max Protocols

1. Resting Metabolic Rate (RMR) Protocol

You relax comfortably in a reclined position for 15 minutes wearing the light PNOĒ facemask. We measure basal fuel consumption at complete rest to determine the exact calories and macro proportions your body utilizes daily without exercise.

2. Active VO2 Max Ramp Protocol

Performed on a lab treadmill or cycle ergometer. The intensity gradually increases in progressive 1-2 minute stages until maximal voluntary exertion. We track your aerobic threshold, lactate transition, and maximum oxygen consumption.

Actionable Takeaways You Receive

  • Identify your accurate Anaerobic Threshold (AT) and Respiratory Compensation Point (RCP)
  • Pinpoint exact heart rate training zones (Zone 1 through Zone 5) instead of formula approximations
  • Calculate exact daily caloric expenditure to fine-tune nutrition for fat loss or lean hyper-trophy
  • Detect hyperventilation syndromes, diaphragmatic breathing dysfunctions, or respiratory fatigue
  • Directly program personalized cardio protocols with your Alpha 1 Personal Trainer
TRAINING APPLICATION
Used by Alpha 1 Personal Trainers for targeted Zone 2 cardio
Alpha 1 Personal Training
CLINICAL MARKER BREAKDOWN

RMR vs. AMR: Side-by-Side Physiological Comparison

Resting Metabolic Rate (RMR) and Active Metabolic Rate (AMR / VO2 Max) measure two fundamentally different physiological states. Together they form the complete picture of how your body burns fuel — at rest and at peak effort.

Resting Protocol

RMR — Resting Metabolic Rate

Quantifies the precise calories and fuel mixture your body consumes at complete rest. The foundation of any accurate nutrition plan — eliminates the guesswork from "how much should I eat?"

Active Protocol

AMR — Active Metabolic Rate (VO2 Max)

Measures peak oxygen consumption and substrate oxidation under graded exercise. Reveals your cardiovascular ceiling, exact heart-rate training zones, and the crossover where fat-burn shifts to glycogen-burn.

Physiological Marker
RMRResting
AMRActive VO2 Max
Primary MeasurementBasal Metabolic Rate (BMR) — calories burned at complete restVO2 Max — peak aerobic capacity & maximal oxygen uptake
Physiological StateFully rested, fasted, reclined, autonomic baselineProgressive graded exertion to maximal voluntary effort
Breath AnalysisResting O2 consumption & CO2 production (VO2 / VCO2 at rest)Breath-by-breath gas exchange across every intensity stage
Respiratory Quotient (RQ)Resting substrate split — fat vs carbohydrate oxidation ratioCrossover point where fat-burning shifts to glycogen-burning
Fat Oxidation RateGrams of fatty acids burned per hour at baselineFatMax — peak fat-burn heart rate & gram/hour ceiling
Daily Caloric NeedExact 24-hour resting energy expenditure (kcal/day)Active + total energy expenditure (kcal/day w/ exercise)
Metabolic EfficiencyHow efficiently mitochondria conserve fuel at restVentilatory Thresholds (VT1 / VT2) & biological age score
Protocol Duration~15 minutes reclined with PNOĒ metabolic mask~10-15 minutes graded ramp on treadmill or Wattbike
Best ForNutrition planning, caloric deficit / surplus, plateau diagnosisHeart rate zones, cardio programming, endurance & VO2 longevity

RMR Alone

Tells you exactly how many calories to eat for fat loss, maintenance, or lean gain — with zero formula estimation.

AMR Alone

Gives you personalized heart-rate zones and a cardio ceiling — the blueprint for endurance and Zone 2 longevity training.

Dual Test (RMR + AMR)

The complete metabolic profile: resting fuel burn + peak capacity + exact training zones. The gold-standard Alpha 1 metabolic blueprint.

Shared methodology: Both tests use the same PNOĒ metabolic analyzer and breath-by-breath gas exchange technology. The difference is physiological state — rest versus graded maximal exertion — which exposes different mitochondrial and cardiopulmonary markers from the same breath data.

TRANSPARENT STANDARD PRICING

PNOĒ ASSESSMENT PRICING

Clinical breath-by-breath cardiopulmonary testing tailored to your metabolic and athletic goals.

45 Minutes

Resting (RMR) Only Test

Fasted morning metabolic test measuring exact basal caloric expenditure and substrate burn.

$175.00/ session
What's included:
  • Fasted breath-by-breath analysis
  • Basal Metabolic Rate (BMR) calculation
  • Fat vs Carbohydrate oxidation at rest
  • Cellular metabolic efficiency score
  • Targeted daily caloric target blueprint
60 Minutes

Active (AMR) VO2 Max Test

Graded exercise treadmill or Wattbike test measuring cardiopulmonary capacity and heart rate zones.

$199.00/ session
What's included:
  • Clinical VO2 Max & biological age score
  • Ventilatory Threshold 1 & 2 (VT1 / VT2)
  • Exact 5 Heart Rate Training Zones
  • FatMax & peak carbohydrate burn zones
  • Respiratory compensation & breath pacing
90 Minutes Total

Dual Test (RMR + AMR)

Complete rest-to-peak-exertion metabolic profiling for nutrition and conditioning precision.

$275.00/ session
What's included:
  • Full Resting (RMR) + Active (AMR) testing
  • Complete 24-hour total energy expenditure
  • Resting substrate split + VO2 max zones
  • Cardiorespiratory fitness benchmarking
  • Integrated training & nutrition roadmap
Most Complete
90-100 MinutesBest Value

Dual Test + InBody Scan

The complete Alpha 1 Gold Standard: full cardiopulmonary breath analysis paired with clinical body composition.

$299.00/ session
Complete Gold Standard Package:
  • Full Resting (RMR) + Active (AMR) testing
  • InBody 270 Segmental Body Composition
  • Visceral fat area & extracellular water ratio
  • Mitochondrial fuel utilization mapping
  • Direct translation into Alpha 1 training plan
Includes both breath analysis & InBody scan report
All tests conducted by certified exercise physiologists using gold-standard PNOĒ diagnostic hardware.
PRECISION LAB SCHEDULING
Gold Standard

Ready to Book Your PNOĒ Metabolic & VO2 Max Assessment?

Clinical breath-by-breath cardiopulmonary exercise testing (CPET) and resting metabolic rate (RMR) analyzing 23+ biomarkers in every breath.

$299.00per session
90–100 Minutes
Secure checkout Flexible scheduling PNOĒ partner
WHAT'S INCLUDED:
  • Full Resting (RMR) + Active (AMR) VO2 Max testing
  • InBody 270 segmental body composition scan
  • Visceral fat area & extracellular water ratio
  • Mitochondrial fuel utilization & fat oxidation mapping
  • Exact 5 heart rate training zones + biological age
  • Integrated Alpha 1 training & nutrition roadmap